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KMID : 0357319920270060517
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 6 p.517 ~ p.524
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Analysis of Gene Products Induced by Phosphate Starvation in Enteric Bacteria
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Abstract
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The regulatory genes of phosphate regulon in pathogenic enteric bacteria were analyzed. As gene products, which can be easily examined, phosphate-binding protein(PBP) and alkaline phosphatase(AP) were selected. The production of PBP and AP was
analyzed
in Klebsiella pneumoniae and Shigella dysenteriae and the results were compared with those from Escherichia coli.
The AP activity of wild type strain of E. coli was five hundred times higher in low phosphate medium than in high phosphate medium. The activity of pho mutant was low in both low phosphate medium and high phosphate medium. The AP activity of phoR
mutant
was high in both low phosphate medium and high phosphate medium.
The AP activity of K. pneumoniae and S. dysenteriae was assayed. The AP activity of K. pneumoniae showed ten-fold induction in low phosphate medium. This suggested that the regulatory genes of K. pneumoniae functioned in basically same manner as
those
of wild type strain of E. coli.
The AP activity of S. dysenteriae showed strain difference. Strains Sh and type 1 showed low AP activities in both low phosphate medium and high phosphate medium. This showed that these two strains were vhoR. PhoM double mutant, since S.
dysenteriae was
known to contain normal phoB gene. The AP activity of strains both type 2 and type 3 of S. dysenteriae showed over one thousand fold induction, which showed that these two strains were same functional genes as those of E. coli.
Another gene product of phosphate regulon, the PBP, which was analyzed by Western blotting in these enteric which proved the interpretation of AP assay results.
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KEYWORD
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